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ROXY9 shows a lower reactivity towards GSSG as compared to GRXC2 To elucidate whether the lack of oxidoreductase activity of ROXY9 on HED, Cys-SG and roGFP2 is caused by a different reactivity towards GSH and/or GSSG as compared to class I GRXs, we subjected strep-MBP-ROXY9 and strep-MBP-GRXC2 to different GSH/GSSG molar ratios at p H 7 and monitored their corresponding redox states using the alkylation shift assay as described above

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