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doi: 10.1186/s13054-025-05283-0 107 ShiZGongSLiYYanKBaoYNingK

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Malic Enzymes (ME) The synthesis of NADPH via the action of malic enzyme is directly coupled to the process of the transport of acetyl-CoA from the mitochondria to the cytosol where the acetyl-CoA can then be used for fatty acid synthesis, cholesterol synthesis, phospholipid synthesis, and deoxynucleotide synthesis as indicated above

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), TRIzol reagent, oligo(dT)18 primers, murine maloney leukemia virus (MMLV) reverse transcriptase, RNase inhibitor, ethidium bromide (EtBr), and agarose were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA)