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El interior de la membrana de las mitocondrias es impermeable a los acetil-CoA por que deben unirse a la carnitina para que el acetil-carnitina resultante sea transportado a la matriz mitocondrial

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After blocking, the membranes were incubated with mouse anti-GST (Santa Cruz Biotechnology), TG2 7 , and FLAG antibodies (Sigma-Aldrich) at 4 C overnight