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It does fade really quickly, especially after drinking coffee

Recent evidence suggests that intentional weight loss in older adults with overweight and obesity is effective and safe, hence a diminishing reluctance to use AOMs in this more vulnerable population

Nat Rev Cardiol 20(7):475494 Yuen MMA (2023) Health complications of obesity: 224 obesity-associated comorbidities from a mechanistic perspective

Deglutamylation activities using -glutamyl- p -nitroanilide as the substrate were determined spectrophotometrically according to a previously described method (Orlowski and Meister, 1963), with slight modifications, as follows: the reaction mixture, which consisted of 0.0125 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- p -nitroanilide, was incubated for 6 h at 37C, and p -nitroaniline released from -glutamyl- p -nitroanilide was monitored at 412 nm