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The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min

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The advent of NGS has significantly shifted the diagnostic approach from a biopsy-first strategy to a genetic-first strategy

Prostaglandin E2 is an endogenous modulator of cerebellar development and complex behavior during a sensitive postnatal period