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Increased cellular protein S -glutathionylation sensitized hepatocytes to TNF hepatotoxicity Increased cellular glutathionylated protein levels in response to GSSG accumulation prompted us to examine the effects of cellular protein S -glutathionylation on TNF-induced cell death

Quantitative real-time PCR (q-PCR) for mRNA level Total RNAs were extracted from MCF-7 and ZR-75-1 cells using RNA-easy Isolation Reagent (Vazyme, Nanjing, China) according to the manufacturers instructions