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This approach focused on glycoprotein nonmetastatic melanoma protein B (GPNMB) as a molecular target for senolytic therapy

9711, otherwise known as the Food and Drug Administration Act of 2009, the manufacture, importation, exportation, sale, offering for sale, distribution, transfer, non-consumer use, promotion, advertising or sponsorship of health products without the proper authorization is prohibited

Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV