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The mixture was shaken for 5 min for thorough mixing and centrifuged at 14,000 rpm for 10 min at 4 C, and the supernatants were directly transferred into specific glass vials until assayed

But the lack of district planning and the failure to acquit how funds are being used raises questions about the proper use of public monies and the effectiveness of the programs intended to improve the lives of citizens

Xiao, J., Zhou, Z., Zhong, G., Xu, T