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Following alignment, the resulting BAM files were sorted and indexed using SAMtools (v1.12)

We evaluated the lipoylation status of the E2 component of the pyruvate dehydrogenase complex (PDH-E2) and dihydrolipoamide S-succinyltransferase (DLST) as surrogate markers for LIAS activity and found no change in protein lipoylation with respect to cysteine availability (Fig

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